PCR Supplies
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Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Reaction Format | Premixed Components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | Cells |
| For Use With (Application) | RT-PCR,Real Time PCR (qPCR) |
Invitrogen™ SuperScript™ IV RT Reaction Buffer
SuperScript IV RT Reaction Buffer is included with, and designed for use with, SuperScript IV Reverse Transcriptase.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ Reagents Starter Kit
TaqMan™ PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan™ 5' nuclease assay with the TaqMan™ PCR Master Mix.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Sample Type | DNA (Genomic), cDNA |
|---|---|
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression |
| Detection Method | Primer-probe |
Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit
TaqMan™ RNase P Detection Reagents Kit
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2 X |
| Form | Liquid |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,DNA Quantification,Presence/Absence,miRNA analysis |
| PCR Method | qPCR |
| Multiplex Capability | Duplex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | AmpliTaq Fast DNA Polymerase |
| Product Type | Fast Advanced Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Inhibitor Tolerance (Simple) | High |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqMan |
Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Content And Storage | Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Fidelity (vs. Taq) | 2 X |
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Product Type | Universal Master Mix II |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| Product Line | TaqMan |
Applied Biosystems™ Power SYBR™ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Purity or Quality Grade | LD (Low DNA) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Product Line | SYBR |
Invitrogen™ M-MLV Reverse Transcriptase Buffer
M-MLV Reverse Transcriptase Buffer is for use with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ AccuPrime™ SuperMix II
AccuPrime SuperMix II is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Purity or Quality Grade | LD (Low DNA) |
| Reverse Transcriptase | M-MLV |
| Purification Time | 5 min. |
| Concentration | 20X |
| For Use With (Application) | RT-PCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Cells |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Hot Start | Built-In Hot Start |
| Product Line | Cells-to-CT, SYBR |
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | AccuPrime GC-Rich DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR |
Applied Biosystems™ SYBR™ Select Master Mix for CFX
A cost-effective solution for real-time PCR applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Form | Liquid |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq DNA Polymerase |
| Format | Vial |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast or Standard |
| Label or Dye | SYBR GreenER |
| Hot Start | Built-In Hot Start |
| Product Line | SYBR |